The effect of temperature on the adhesion of cultured Chinese hamster lung (CHL) cells.

Cell adhesion is a multistep process involving initial contact of the cell with the surface, cell attachment and spreading on the surface, and cell growth (1.2). To determine whether cell adhesion is an active or passive phenomena, initial cell attachment assays have previously been carried out either at various temperatures (3) or in the presence of metabolic inhibitors (4). However, all of these studies are concerned only about initial cell atttachment. In this communication we report the results of a study relating the attachment of cells and their adhesion strength, at various temperatures between 4°C and 37°C.